Separation ofParacoccidioides brasiliensisconidia through Percoll gradients
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چکیده
منابع مشابه
Comparison of CapriPure and Percoll density gradients for sperm separation of frozen- thawed goat spermatozoa
Frozen-thawed semen from six Boer bucks was pooled and used to compare the recently available commercial product CapriPure with Percoll for sperm quality. Sperm quality parameters such as sperm motility, concentration, membrane integrity (PI/CFDA), acrosome integrity (FITC-PNA) and mitochondrial activity (JC-1) were evaluated before and after sperm processing using CapriPure and Percoll density...
متن کاملEffects of centrifugation through three different discontinuous Percoll gradients on boar sperm function.
In this study, different combinations of 2-step, discontinuous gradient centrifugation were used, consisting of three different combinations of isotonic Percoll (45/60, 60/75 and 45/90%) that allowed us to select different sperm subpopulations from fertile and normozoospermic boars. Our objective in this study is to evaluate the effects of centrifugation through three different discontinuous Pe...
متن کاملSynchronization of cell division in microorganisms by percoll gradients.
We describe a method for obtaining synchronously dividing cells of bacteria (Escherichia coli B and K-12 and Bacillus subtilis 168) and fission yeasts (Schizosaccharomyces pombe) by the use of Percoll density gradients.
متن کاملDistribution of chromaffin secretory vesicles, acetylcholinesterase, and lysosomal enzymes in sucrose and Percoll gradients.
Crude chromaffin secretory vesicles, obtained by differential centrifugation, were further purified on isotonic (Percoll) gradients. The chromaffin vesicle fractions recovered from the gradients contain acetylcholinesterase as well as lysosomal enzymes. With the aid of a subsequent sucrose gradient lysosomal enzymes could be removed from chromaffin vesicle fractions, but not acetylcholinesteras...
متن کاملIsolation of brain and spinal cord mononuclear cells using percoll gradients.
Isolation of immune cells that infiltrate the central nervous system (CNS) during infection, trauma, autoimmunity or neurodegeneration, is often required to define their phenotype and effector functions. Histochemical approaches are instrumental to determine the location of the infiltrating cells and to analyze the associated CNS pathology. However, in-situ histochemistry and immunofluorescent ...
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ژورنال
عنوان ژورنال: Medical Mycology
سال: 2004
ISSN: 1369-3786,1460-2709
DOI: 10.1080/13693780410001657126